目的:回顾性分析本院患儿九项呼吸道病原体感染的分布情况,为临床诊疗提供依据。方法:选取2023年1月~2023年12月至我院门诊就诊或住院治疗的748例急性呼吸道感染患儿作为研究对象,采用间接免疫荧光法对所有患儿的血清标本进行九项呼吸道病原体检测及分析。结果:748例患儿血清标本中共检测出阳性例数为281例,总阳性率为37.57%。其中男性患儿的阳性率为19.25%,女性阳患儿的阳性率为18.32%,两者差异无统计学意义(P > 0.05)。在单一呼吸道病原体阳性的患儿中,主要以肺炎支原体最常见,占阳性例数的27.14%,其次为副流感病毒和呼吸道合胞病毒,分别为3.07%和0.94%;混合感染有44例,占感染比例的5.88%。结论:儿童呼吸道感染主要是以肺炎支原体为主,其次为副流感病毒和呼吸道合胞病毒,及时检测出患儿呼吸道感染病原体的类型,为临床对患儿的诊疗具有重要的指导意义。Objective: To retrospectively analyze the distribution of nine respiratory tract pathogen infection in children, so as to provide evidence for diagnosis and treatment. Methods: 748 children with respiratory tract infection admitted into from January 2023 to December 2023 were selected as study subjects. All serum specimens of all children were tested by nine joint inspections of respiratory pathogens and analyzed. Results: Among the 748 patients, 281 were positive, with a positive rate of 37.57%, which that 19.25% were male children and 18.32% were female children and there was no significant difference statistically (P > 0.05). Mycoplasma pneumoniae (MP) was the most common among the children who were positive for single respiratory pathogens, accounting for 27.14% of the positive cases, followed by parainfluenza virus (HPIV) and respiratory syncytial virus (RSV), with a positive rate of 3.07% and 0.94%, respectively. There were 44 cases of mixed infection, accounting for 5.88% in all the positive patients. Conclusion: MP is the