Objective To establish a uremic apoE-/- mouse model to observe serum biochemical parameters and features of aortic root atherosclerosis (AS) in the model. Methods A uremic model was induced surgically in apoE-/- mice: electrocautery of the right kidney at 8 weeks of age and nephrectomy (NX) of the left one 2 weeks later. Control mice were sham-operated. Two weeks after NX,renal functions were detected in the uremic and control mice to evaluate the efficiency of the model. After 10 weeks of NX,blood samples were taken to determine serum biochemical parameters,and aortic root was collected for frozen sections to investigate the lesion features of AS. Results Two weeks after NX,renal functions declined significantly in the uremic mice compared with the control ones,and remained stable 8 weeks later either in males or in females. Ten weeks after NX,serum levels of TCH,TG and LDL-C were dramatically higher in the uremic mice than in the controls,whereas no differences in serum HDL-C or glucose concentration were found between the two groups. Aortic root plaque relative area increased significantly in the uremic mice compared with the controls either in males or in females; more-over,the lesion area was larger in female mice than in male ones. Conclusion We established a uremic apoE-/- mouse model successfully,and this model is characterized by accelerated atherogenesis which is associated with an increase in serum lipid profile. This experimental model can be a useful tool to study the pathogenesis and therapeutic strategies of uremic AS.
Yan Shen1,Zu-Yi Yuan2,Yan Xiao3,Li-Jun Wang2,Yue Wu2,Xiao Liang2,Yan Zhao2,Yu-Ling Tian2,Wei-Min Liu2,Tao Chen21. Department of Nephrology,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061
目的探讨急性冠脉综合征(ACS)患者外周血辅助性T细胞亚群(Th1/Th2)的变化及意义。方法采用流式细胞术和实时定量PCR的方法检测22例急性心肌梗死(AMI)、25例不稳定型心绞痛(UA)、20例稳定型心绞痛(SA)患者和25例正常对照外周血Th1/Th2细胞频率及相关基因T-bet、STAT4(signal transducer and activator of transcription4)、干扰素γ(inter-feronγ,IFN-γ)、GATA3和白介素-4(interleukin-4,IL-4)mRNA的表达。结果①与SA组和对照组相比,ACS组(包括UA组和AMI组)患者外周血中Th1细胞频率显著增高(P<0.01),而Th2细胞频率的变化无统计学意义;②ACS组(包括UA组和AMI组)患者Th1细胞相关基因T-bet、STAT4和IFN-γ的mRNA表达显著高于SA组和对照组(P<0.01),而Th2细胞相关基因IL-4和GATA3的mRNA表达在各组之间未见明显差异。结论 Th1/Th2失衡可能参与急性冠脉综合征的发生发展。
