In 2006, the indica photoperiod- and thermo-sensitive genic male sterile (PTGMS) line Yan 161S was successful y bred by crossing the Guangzhan 63S (♀) and the medium indica breeding material Yan 229 (♂), which was bred by the Institute of Agricultural Sciences in Coastal Region of Jiangsu Province, after years' and generations’ selection in different areas. It passed the identification by Jiangsu Provincial Crop Variety Appraisal Committee in 2013. Crossing the Yan 161S with Yanhui 888, a new high-yielding and high-quality two-line hybrid rice combination Yanliangyou 1618 was developed, and it passed the variety identification in 2014. This paper introduced the breeding processes and characteristics of Yan 161S and Yanliangyou 1618 and the cultivation and seed production techniques of Yanliangyou 1618.