[Objective] To extract the effective component rosmarinic acid from Folium perillae,study preparation technology and quality control of rosmarinic acid injection and provide test basis for hemorheological drug development and clinic application.[Method]The coarse powder of Folium perillae(40 μm)was lixiviated with hot water and acidized to make aqueous extracts.Rosmarinic acid from the aqueous mixture was extracted with Ethyl Acetate.And Et Ac was evaporated to obtain primary product.The injection was purified by pH adjustment.Concentration of rosmarinic acid in the injection was detected by high performance liquid chromatography(HPLC).Contents of tannin,resin and oxalate were eliminated according to the Chinese Pharmacopeia(2005 edition).Stability,irritation,haemolyticus,LD50 and thermogenic substance of this injection were also detected.[Result]The content of rosmarinic acid in Folium perillae injection is 2.81 mg/ml,and the content of LD50 was 406.82 mg/kg.This injection was consistent with the stipulation of Chinese Pharmacopeia(2005 edition).[Conclusion]The preparation technology of this experiment was reasonable.The stable qualities of the prepared injection meet the injection requirement.
[ Objective ] Study on the effect of perilla seed on blood lipid regulation and peroxidation of Coturnix coturnix with hyperlipidemia. [ Method ] The high-lipid.diet was fed to establish the high lipid model of Coturnix coturnix. The normal control group, high lipid model group, high dose of perilla seed group（2.5 g/kg）, low dose of perilla seed group （0.83 g/kg） were designed to measure the contents of total cholesterol （TC）, three acids glyceride （TC）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, very low density lipoprotein cholesterol （VLDL-C） and malondialdehyde （MDA）, superoxide dismutase （SOD） activity. [ Result] All the test groups could decrease TC ,TG,LDL-C and MDA content and significantly increase the HDL-C content （p 〈 0.01） and SOD activity（p 〈 0.01 ） in serum. [ Conclusion] Perilla seed has the blood lipid regulation and peroxidation function.
