通过观察镁离子对紫外线辐射损伤后细胞活性与光产物产量的影响,以明确镁离子对UVB辐射细胞损伤的防护作用。在实验中,选择成人成纤维细胞(HDF-a)和角质形成细胞(HaCaT)进行细胞培养,筛选小剂量UVB辐射进行诱导性损伤,观察不同浓度镁离子(0.1 mm ol/L,0.2 mm ol/L,0.4 mm ol/L,0.8 mm ol/L,1.2 mm ol/L)对辐射损伤的细胞活性、基因组断裂情况及光产物产量的影响。研究结果显示,在UVB辐射后,两个细胞系细胞活性显著下降,镁离子培养液孵育的细胞活性均比UVB辐射损伤对照组显著升高,而镁离子孵育后细胞光产物嘧啶二聚体(CPD s)量明显减少。实验研究证实,镁离子对UVB辐射损伤的培养细胞具有防护作用,为UV辐射所致的DNA损伤的修复提供理论基础。
Using the optical coherence tomography(OCT) images,the optical attenuation coefficients(μ_t) of human Laogong acupoint and non-acupoint tissues are measured after empty irradiation and 808-nm 100-mW irradiation for 10 min in vivo non-invasively.The results show that there is no significant difference ofμ_t of Laogong acupoint and non-acupoint tissues after empty irradiation.However,there are significant differences ofμ_t between Laogong acupoint and non-acupoint tissues after laser irradiation at the power of 100 mW(statistical definition of probability p<0.01).The results of the pilot study indicate that the OCT could distinguish the acupoint from the surrounding tissues after irradiation with laser in vivo non-invasively.
