Plants have an ability to flower under optimal seasonal conditions to ensure reproductive success.Photoperiod and temperature are two important season-dependent factors of plant flowering.The floral transition of plants depends on accurate measurement of changes in photoperiod and temperature.Recent advances in molecular biology and genetics on Arabidopsis and rice reveals that the regulation of plant flowering by photoperiod and temperature are involved in a complicated gene network with different regulatory pathways,and new evidence and understanding were provided in the regulation of rice flowering.Here,we summarize and analyze different flowering regulatory pathways in detail in rice based on previous studies and our results,including short-day promotion,long-day suppression,long-day induction of flowering,night break,different light-quality and temperature regulation pathways.
Photoperiod and temperature are two pivotal regulatory factors of plant flowering. The floral transition of plants depends on accurate measurement of changes in photoperiod and temperature. The flowering time of rice （Oryza sativa） as a facultative short-day （SD） plant is delayed under long-day （LD） and/or low temperature conditions. To elucidate the regulatory functions of photoperiod and temperature on flowering time in rice, we systematically analyzed the expression and regulation of several key genes （Hd3a, RFT1, Ehdl, Ghd7, RID1/Ehd2/OsIdl, Se5） involved in the photoperiodic flowering regulatory pathway under different temperature and photoperiod treatments using a photoperiod-insensitive mutant and wild type plants. Our re- sults indicate that the Ehdl-Hd3a/RFT1 pathway is common to and conserved in both the photoperiodic and temperature flow- ering regulatory pathways. Expression of Ehdl, Hd3a and RFT1 is dramatically reduced at low temperature （23~C）, suggesting that suppression of Ehdl, Hd3a and RFT1 transcription is an essential cause of delayed flowering under low temperature con- dition. Under LD condition, Ghd7 mRNA levels are promoted at low temperature （23~C） compared with normal temperature condition （28℃）, suggesting low temperature and LD treatment have a synergistic role in the expression of Ghd7. Therefore, upregulation of Ghd7 might be a crucial cause of delayed flowering under low temperature condition. We also analyzed Hdl regulatory relationships in the photoperiodic flowering pathway, and found that Hdl can negatively regulate Ehdl transcription under LD condition. In addition, Hdl can also positively regulate Ghd7 transcription under LD condition, suggesting that the heading-date of rice under LD condition is also regulated by the Hdl-Ghd7-Ehdl-RFT1 pathway.
