Based on chemical thermodynamics and mass balance,countercurrently fractional chiral extraction by hollow fiber membrane was investigated.The mathematical modules for the relation of chiral extraction yield and product optical purity with number of transfer units by chiral extraction with hollow fiber membrane were established,and the modules were simulated by visual basic(VB)proceduce.The results show that,the difference in free energy between two diastereomeric complexes formed by R-and S-enantiomer with chiral selector,??(?G),is the force of separation of enantiomers.It is necessary to separate enantiomers,where one of the extraction factors is above 1,and the other is below 1.Under certain phase ratio,chiral separation depends on separation factor and number of transfer units.The experimental result is in agreement with the theoretical value.
A series of extractants (tert-butylcalix[6]arene, tert-butylcalix[8]arene and octeacetate of tert-butylcalix[8]arene) were synthesized, and their structures were identified by IR and 1H-NMR. The distribution behavior of ester catechins monomer in the aqueous and chloroform two-phase system containing one of calixarene was studied. The influences of different extractants, concentration of tert-butylcalix[8]arene and extraction temperature on the partition coefficients and the separation factors were investigated. The experiment results show that tert-butylcalix[8]arene is the best extractant that forms a more stable supramolecular compound with gallocatechin gallate (GCG) than with epigallocathechin gallate (EGCG) or epicatechin gallate (ECG). When the concentration of p-tert-butylcalix[8]arene is 3.79 mmol/L, the extraction temperature is 4 ℃, the partition coefficients of KGCG, KECG, KEGCG are 0.987, 0.629, 0.449,the separation factors of α1 and α2 are 1.450 and 1.596, respectively. The important factors influencing the extraction properties of calixarene are discovered to be its cavity size and hydrogen bonding.
