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国家自然科学基金(30230190)

作品数:7 被引量:42H指数:4
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发文基金:国家自然科学基金国家重点基础研究发展计划中国科学院知识创新工程重要方向项目更多>>
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我国男性避孕研究的发展前景被引量:16
2004年
男性避孕研究长期未得到足够重视。文中综述了国内外相关研究的最新进展。重点评述了我国在用棉酚、长效睾酮和温浴睾丸作为抗精子发生所取得的成就和存在的问题,提出了“睾丸局部温浴组合低剂量棉酚”,“长效睾酮组合低剂量棉酚”和“睾丸局部温浴组合缓释长效睾酮”3种不同组合的临床研究方案和理论依据。对睾丸局部热压主要促进Ⅰ-Ⅳ期和Ⅸ-ⅩⅢ精细胞凋亡,长效睾酮主要促使Ⅶ-Ⅷ期的精细胞凋亡,而低剂量棉酚除对睾丸变态精子有作用外,主要影响附睾精子。这些组合各作用在精子发生的不同阶段,通过不同的信息通路作用于精子发生和形成的不同位点,优势互补,可大大降低单独应用时的剂量、作用时间和强度,避免各自使用时的副作用,产生最大的组合效应,符合安全、有效和可逆的理想结果。
刘以训
关键词:男性避孕精子发生棉酚
Molecular basis of cryptorchidism-induced infertility被引量:9
2010年
Artificial cryptorchidism or local testicular heat treatment can induce reversible oligospermia or azoospermia in monkeys and rats via germ cell apoptosis. Local warming of monkey testes in water at 43°C for 2 consecutive days (30 min per day) decreased the number of sperm in the semen by up to 80% on d 28, and the effect was completely reversed on d 144. Germ cells rely heavily on Sertoli cells for structural and nutritional support. Specialized junctions that play a pivotal role in spermatogenesis occur at sites of Sertoli-Sertoli and Sertoli-germ cell contact in the seminiferous epithelium. We demonstrated that expression of tight junction (TJ)-associated molecules, such as occludin and zonula occludens-1 (ZO-1), were greatly reduced 24–48 h after heat treatment, while the permeability of the blood-testis barrier (BTB) was simultaneously increased, but recovered 10 d later. These results indicate a reversible disruption of the BTB associated with transient inductions of transforming growth factor (TGF) β2 and β3 expression, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase activation, and concomitant loss of occludin and ZO-1. This suggests that expression of TJ-associated molecules and the BTB was reversibly perturbed by mild testicular hyperthermia, and that the heat-induced induction of TGF-β might be involved in downregulating TJ-associated proteins, leading to cell junction reduction. This review discusses the changes in total gene expression patterns after experimental cryptorchidism in adult mouse testes, and the cloning of several novel, physiologically significant spermatogenesis-specific genes.
LIU YiXun & LI XiXia State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
关键词:CRYPTORCHIDISMSPERMATOGENESISSERTOLITIGHTJUNCTION
Activation of extracellular signal-related kinases 1 and 2 in Sertoli cells in experimentally cryptorchid rhesus monkeys被引量:6
2006年
Aim: To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases (MAPK) in response to heat stress in the cryptorchid testis, and to investigate a possible relation to Sertoli cell dedifferentiation. Methods: Immunohistochemistry and western blot were used to examine the expression and activation of ERK1/2, p38 and JNK in the cryptorchid testis at various stages after experimental cryptorchidism. Results: The abdominal temperature did not obviously change the total ERK1/2 expression but significantly activated phospho-ERK1/2 in the Sertoli cells of the cryptorchid testis. Heat stress increased total JNK expression in the Sertoli cells of the cryptorchid testis but did not activate phospho-JNK. Neither total p38 nor phospho-p38 was induced by heat stress in the Sertoli cells of the cryptorchid testis. Changes in the spatiotemporal expression of cytokeratin 18 (CK18), a marker of immature or undifferentiated Sertoli cells, were induced in the cryptorchid testis in a pattern similar to the activation of ERK1/2. Condusion: The activation of ERK1/2 in the testis may be related to dedifferentiation of Sertoli cells under heat stress induced by experimental cryptorchidism.
Xue-Sen Zhang Zhi-Hong Zhang Shu-Hua Guo Wei Yang Zhu-Qiang Zhang Jin-Xiang Yuan Xuan Jin Zhao-Yuan Hu Yi-Xun Liu
关键词:CRYPTORCHIDISMDEDIFFERENTIATION
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