ORFⅡ gene of Banana streak virus GuangDong isolate(BSV-GD) was amplified from a BSV-GD recombinant plasmid by PCR,and the gene was expressed by being cloned into prokaryote expression vector pET-28b(+).The fusion protein was about 16.5 kDa in size and was soluble with SDS-PAGE analysis.The purified protein was obtained by using the histidine labeling kit of N-terminus of protein.The antiserum was obtained by immunizing healthy rabbits with the purified protein.Western blot and ELISA analysis showed that the special antiserum of BSV possessed high titer,which was tested as 1∶51 200.The study was a base for further research on BSV including ORFⅡ gene function and virus detection.
Rice gall dwarf virus(RGDV)is an important rice pathogen in China and Southeast Asia.However,little is known about the molecular mechanisms of RGDV interactions with plant cells.Here,we have identi-fied an RGDV protein,Pns11,which acts as a suppressor of RNA silencing in coinfiltration assays with the reporter,green fluorescent protein(GFP)in transgenic Nicotiana benthamiana line 16c carrying GFP.Pns11 suppressed local and systemic silencing induced by sense RNA.The spread of mobile RNA si-lencing signals was blocked or inactivated by Pns11.Expression of Pns11 also enhanced Potato virus X pathogenicity in N.benthamiana.This suppressor could reduce,but not eliminate,siRNA in the local and systemic RNA silencing suppression assays,suggesting that Pns11 functions by interfering with initial stages of RNA silencing.
