Some unique subclasses of Camelidae antibodies are devoid of the light chain,and the antigen binding site is comprised exclusively of the variable domain of the heavy chain(VHH).The recombinant VHHs have a high potential as alternative reagents for the next generation of immunoassay.In particular,they might be very useful for molecular mimicry.The present study demonstrated an alpaca immunized with the F(ab’)2fragment of anti-aflatoxin B1 mAb and developed an important anti-idiotypic(anti-Id)responses.Antigen-specific elution method was used for panning private anti-Id VHHs from the constructed alpaca VHH library.The selected VHHs were expressed,renatured,purified,and then identified by a competitive enzyme-linked immunosorbent assay(ELISA).Our findings indicated that the VHH would be an alternative tool for haptens mimicry studies.
WANG DanXU YangTU ZhuiFU Jin HengXIONG Yong HuaFENG FanTAO YongLEI Da
