Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence,840 bp of cp gene and 211 bp of 3′-untranslated region of the isolate ZYMV-Liaocheng was determined.The cp gene of ZYMV-Liaocheng shared identities of 81.4%-98.8% and 89.4%-99.5% at nucleotide and amino acid levels,respectively,with other ZYMV sequences available in the GenBank.Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes.ZYMV-Liaocheng belonged to genotype I,which contained isolates from Asia,Europe and America.Genotypes III and V were unique and contained only isolates from East Asia.The isolates from East Asia had the highest variability.
Peanut mottle virus(PeMoV) was detected via RT-PCR from two peanut samples(QD5 and QD6) with mottle symptom collected from Qingdao,Shandong Province.The 3′-terminal 892 bp fragments of their genome were cloned and sequenced.The cp genes of QD5 and QD6 were 837 bp in length and encoded 278 amino acids(aa),with DAA at aa sites regulating aphid transmission.QD5 and QD6 shared nucleotide identities of 95.3%-99.4% and aa identities of 93.5%-99.6% in cp genes with other PeMoV isolates available in the GenBank.The phylogenetic results showed that PeMoV were clustered to three groups,America,Asia and Australia,which were consistent with their geographical origins.This is the first molecular evidence on the incidence of PeMoV in China.
