您的位置: 专家智库 > >

国家自然科学基金(3500521)

作品数:1 被引量:1H指数:1
发文基金:国家自然科学基金更多>>
相关领域:医药卫生更多>>

文献类型

  • 1篇中文期刊文章

领域

  • 1篇医药卫生

主题

  • 1篇CELL_A...
  • 1篇CELL_C...
  • 1篇CELL_P...
  • 1篇GENE_S...
  • 1篇GLIOMA
  • 1篇GLIOMA...

传媒

  • 1篇Journa...

年份

  • 1篇2009
1 条 记 录,以下是 1-1
排序方式:
Effects of RNAi-mediated Gene Silencing of LRIG3 Expression on Cell Cycle and Survival of Glioma Cells被引量:1
2009年
Summary: The effects of RNAi-mediated gene silencing of LR1G3 expression on cell cycle and survival of human glioma cell line GL15 and the possible mechanisms were explored. The plasmids pGenesil2-LRIG3-shRNA1 and pGenesil2-LRIG3-shRNA2 were transfected into GLI 5 glioma cells respectively by using Metafectine, and the transfected cells that stably suppressed LR1G3 expression were selected by G418. The control cells were transfected with negative control shRNA. The changes in LRIG3 mRNA and protein levels were measured by RT-PCR and Western blot. The apoptosis rate and cell cycle were analyzed by flow cytometry. As compared with the negative shRNA-transfected GL 15 cells, LRIG3 mRNA expression in GLI 5 cells transfected with pGenesil2-LRIG3-shRNA 1 and pGenesil2-LRlG3-shRNA2 was silenced by 52.4%, 63.8%, and LRIG3 protein expression was reduced by 50.9% and 67.4% respectively. The LRIG3-specific siRNA transfected cells had higher proliferation rate than control cells. Cell cycle analysis showed that silencing LRIG3 increased the percentage of G2/M phase cells and the proliferation index significantly (P〈0.01). Silencing LRIG3 could inhibit the apoptosis of GL15 cells (P〈0.05). These findings suggest that the siRNA targeting LRIG3 gene shows a dramatic inhibitory effect on RNA transcription and protein expression, then promoting the proliferation of GL15 cells, arresting GL15 cells in G2/M phase, and suppressing apoptosis of GL15 cells.
蔡明俊谢蕊繁韩林陈如东王宝峰叶飞郭东生雷霆
关键词:GLIOMA
共1页<1>
聚类工具0