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国家公益性行业科研专项(nyzx07-024)

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纤维素酶活测定影响因素的研究被引量:30
2010年
研究和分析了3,5-二硝基水杨酸法测定羧甲基纤维素酶糖化力的实验条件,尤其是对测定波长的选择、底物和酶液加量、空白样的选择、酶液稀释倍数对酶活的影响等方面进行了创新性的探究;另外对常规性的影响因素:酶促反应时间、反应pH、底物的浓度等传统因素做了研究。实验表明:在40℃,pH4.6,底物浓度为10g/L,底物和酶液加量各2mL,酶促反应时间5min,空白样选择终止空白,测定波长480nm处测定OD值比较适宜。
姜心陈伟周波黄坤勇韩明渠
外源苯甲酸对平邑甜茶幼苗根系膜脂过氧化的影响被引量:11
2009年
Malus hupehensis was used as experiment materials.This experiments were conducted to explore the effects of benzoic acid on the productivity of superoxide(O2 ·),the contents of malondialdehvde(MDA)and sulfhydryl group activity(—SH),antioxidant enzyme activity and mineral elements in roots of M.hupehensis seedlings.The results showed that there was no significant difference between the treatment of 5 mg·kg-1(soil)benzoic acid and CK in above parameters.With 25 mg·kg-1(soil)and 125 mg·kg-1(soil)benzoic acid treatments,O2 · and MDA contents increased in the early period and then declined;—SH content decreased during the treatment time;activities of SOD and POD increased firstly,then decreased,CAT activity decreased firstly and then slightly reversed,but lower than that of CK;SOD,POD and CAT had a similar trend,activities of roots increased under 5 mg·kg-1(soil)benzoic acid,while they were inhibited by 25 mg·kg-1(soil)and 125 mg·kg-1(soil)benzoic acid;mineral elements were decreased.Benzoic acid in low concentration promoted antioxidant enzyme activity,—SH content,and uptake of mineral elements,reduced O2 · and MDA contents.In conclusion,benzoic acid of 5 mg·kg-1(soil)had no effects on Membrance Lipid Peroxidation and Antioxidant enzymes in roots of M.hupehensis seedlings;at 25 mg·kg-1(soil)and 125 mg·kg-1(soil),benzoic acid increased Membrance Lipid Peroxidationd,decreased activity of antioxidant enzymes of roots,and damaged integrity and stability of cell membrane in M.hupehensis seedlings,meanwhile,contents of mineral elements were decreased.
高相彬胡艳丽赵凤霞毛志泉沈向杨树泉苏立涛
关键词:苯甲酸平邑甜茶超氧阴离子膜脂过氧化抗氧化酶
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