目的获得12个Y染色体短串联重复序列(Y chromosome short tandem repeat,Y-STR)位点(DYSl9、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS385a、DYS385b、DYS437、DYS438、DYS439)在宁夏回族群体的多态性分布。方法应用PowerPlex Y荧光标记复合扩增试剂盒,对宁夏回族群体150名无关健康男性个体基因组DNA进行复合扩增,用ABl377测序仪对扩增产物进行检测,统计12个Y-STRs位点群体遗传学参数。结果12个基因座共检测出75个等位基因,频率分布在0.0067~0.7067之间,基因多样性(gene diversity,GD)分布在0.4446~0.8877之间。在150名无关个体中,共有148种不同的单倍型,其中只有两种单倍型分别为两名个体共有,12个Y-STRs位点联合构成的单倍型多样性为0.9864。结论12个Y-STRs基因座在宁夏回族群体具有较强的个体识别能力,可应用于群体遗传学及法医学研究。
MgAl type layered double hydroxide(LDH) obtained by co-precipitation,has been used as precursors to intercalate an antineoplastic agent fluorouracil to prepare an intercalation compound LDH-FLU with ion exchange reaction.The transformation in structure and crystalline state property from LDH to LDH-FLU has been investigated via XRD,FT-IR,TEM,TG-DSC,and BET-N2 surface area characterizations.The results show that the structure of the intercalation compound is homologous to a monolayer parallel pillar-layer interaction model.Compared with LDH,the crystallinity of this compound is enhanced,the proportion of the inter-lamellar constitution H2O is increased,the thermal decomposition of intermediate crystalline phase complicated,the stack order of grains intensified in c axial,the absorbability of micro-pore decreased,and the pore volume of inter-laminar are reduced evidently.