[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.
