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河北省自然科学基金(C2009001151)

作品数:6 被引量:11H指数:2
相关作者:薛晓英张歌周欢娣杨晔杨玉更多>>
相关机构:河北医科大学第二医院更多>>
发文基金:河北省自然科学基金国家自然科学基金更多>>
相关领域:医药卫生生物学航空宇航科学技术更多>>

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NF-κB与放射线诱导的人食管癌细胞上皮间质转化间的关系被引量:1
2016年
目的探讨NF-κB与放射线诱导的人食管癌细胞上皮间质转化(EMT)间的关系。方法累积照射食管癌Eca109细胞60 Gy,得到Eca109R60细胞,克隆形成实验检测其放射抵抗性,显微镜下观察细胞形态学变化。Real-time PCR和免疫细胞化学检测Eca109和Eca109R60细胞NF-κB、E-cadherin和Vimentin的表达情况。结果 Eca109R60细胞具备了EMT样表型且放射抵抗性更强。Eca109R60细胞中E-cadherin m RNA表达明显下调(P=0.003),Vimentin、NF-κB m RNA表达升高(P=0.004,P=0.004)。相关性分析显示NF-κB与E-cadherin m RNA表达呈负相关,与Vimentin未见有明显相关性,但已显示出正相关的趋势。免疫细胞化学法检测Eca109R60细胞E-cadherin阳性染色程度明显减弱,Vimentin、NF-κB p65阳性染色程度明显增强。结论 Eca109R60细胞放射抗性可能由EMT与NF-κB信号通路相互作用调控。
杨晔薛晓英冉玉格周欢娣张歌肖志清刘军领
关键词:食管癌NF-ΚBE-钙黏蛋白波形蛋白
神经营养因子受体亚细胞定位改变参与食管癌细胞上皮间质转化及放射抗性的形成被引量:1
2016年
目的探讨神经营养因子受体(NRAGE)亚细胞定位改变与人食管癌细胞上皮和间质转化及放射抗性形成的关系。方法应用转化生长因子(TGF—β1)诱导人食管癌细胞TE13,建立上皮间质转化(EMT)模型细胞。以TE13细胞、EMT模型细胞、放射抗拒TE13R120细胞为研究对象,通过Real—timePCR和Westernblot检测EMT标记物mRNA和蛋白表达,验证EMT模型的建立和TE13R120存在EMT样表型。Real-timePCR比较NRAGE在3组细胞中mRNA水平的表达情况,Westernblot检测3组细胞中NRAGE总蛋白及胞质、胞核蛋白的表达。结果TGF—β1诱导TE13细胞形态向间质型细胞转化,EMT标志物E—cadherin和Vimentin表达发生改变(t=13.56、-232.84,P〈0.05),成功建立EMT模型细胞。放射抗拒TE13R120细胞中EMT标记物表现出与EMT模型细胞相似的趋势(t=15.84、-54.54,P〈0.05)。NRAGE在EMT模型细胞、TE13R120细胞中的表达较TE13细胞明显上调,且均在细胞核内表达明显升高(t=-8.73、-5.62,P〈0.05),而在细胞质中表达差异无统计学意义。NRAGE在EMT模型细胞和TE13R120细胞胞质、胞核中的表达差异均无统计学意义。结论放射抗拒TE13R120细胞存在EMT样表型,且其放射抗性的形成可能因EMT的发生参与了NRAGE亚细胞定位的改变而导致。
张亚芳张歌常晓静周欢娣盖晓惠薛晓英
关键词:食管鳞状细胞癌神经营养因子受体上皮间质转化
Relationship between NRAGE and the radioresistance of esophageal carcinoma cellline TE13R120被引量:5
2010年
Background and Objective: The mRNA levels of 59 genes, detected by cDNA microarray, were up-regulated in the radioresistant human esophageal cacinoma cell line TE13R120 as compared with its parental cell line TE13 before and after radiation, and the expression of NRAGE gene showed a gradually up-regulating tendency. This study aimed to further detect the differences of NRAGE gene and protein expression and apoptosis between TE13R120 and TE13 cells, and to investigate the relationship between the NRAGE and the radioresistance of TE13R120 cells and its mechanism. Methods: The two cell lines were irradiated by 60 Co γ-ray at different conditions. Reverse transcription- polymerase chain reaction (RT-PCR), Western blot, and immunocytochemistry were used to detect the expression of NRAGE. Flow cytometry (FCM) was used to detect the cell apoptosis before and after irradiation. Results: The mRNA level of NRAGE was higher in TE13R120 cells than in TE13 cells before and after irradiation (before radiation: 0.25 ± 0.03 vs. 0.49 ± 0.03; 4 Gy 4 h: 0.31 ± 0.03 vs. 0.53 ± 0.02; 4 Gy 16 h: 0.32 ± 0.04 vs. 0.59 ± 0.04; 4 Gy 24 h: 0.36 ± 0.05 vs. 0.72 ± 0.04; 2 Gy 12 h: 0.32 ± 0.02 vs. 0.64 ± 0.04; 6 Gy 12 h: 0.36 ± 0.02 vs. 0.79 ± 0.05; 10 Gy 12 h: 0.46 ± 0.04 vs. 0.85 ± 0.01; P < 0.01), and the mRNA level of NRAGE was increased gradually with the increase of radiation dose and time in the two cell lines (P < 0.05 and P < 0.01). Western blot results showed no difference of NRAGE protein level in cytoplasm between TE13R120 cells and TE13 cells before and after irradiation, but its level in nuclei was higher in TE13R120 cells than in TE13 cells at different radiation time and dosages. Immunocytochemistry showed similar results as Western blot. FCM showed no significant difference in apoptosis rate between TE13R120 and TE13 cells before and after radiation. Conclusion: NRAGE may play an important role in the radiation responses of the two cell lines, and may participate in the formation of radioresistance of TE13R120
Xiao-Ying XueZhi-He LiuFeng-Min JingYan-Ge LiHui-Zhi LiuXian-Shu Gao
关键词:逆转录聚合酶链反应MRNA水平免疫细胞化学GE基因芯片检测
反复照射建立食管癌放射抗性细胞系方法的重复性和稳定性研究被引量:2
2015年
目的探讨射线反复照射方法建立食管癌放射抗性细胞系的重复性及稳定性,并观察辐射抗性细胞与其亲代细胞之间的放射敏感性的差异。方法应用射线对人食管鳞状细胞癌细胞TE13进行反复照射,累计剂量120 Gy,建立具有放射抗性的细胞系TE13R120。采用细胞克隆形成实验测定2种细胞的放射生物学参数,检测其辐射抗性,采用单击多靶模型拟合存活曲线。经连续8 d培养细胞,绘制2种细胞的生长曲线并用Logrank检验计算群体倍增时间。比较此次实验结果与初次建系时结果的相似性。结果接受120 Gy总剂量照射后,TE13R120较TE13表现出明显的放射抗性,TE13R120的放射生物学参数D_0、Dq、N均高于TE13(2.36、2.17、2.50 vs 1.90、1.11、1.80),SF_2、α/β均低于TE13(0.53、2.67 vs 0.73、8.00)。TE13R120的细胞群体倍增时间为20.70 h,长于亲代TE13(17.67 h)。该结果与此前初次建系时结果相似。结论应用射线反复照射并逐步筛选建立辐射抗性细胞系的方法可靠,能建立具有稳定辐射抗性表型的细胞系。
杨延灵薛晓英冉玉格张玉峰李锋杨晔
关键词:食管肿瘤辐射耐受性集落形成单位测定
microRNAs regulation and its role as biomarkers in diseases
2016年
MicroRNAs (miRNAs), approximately 21 to 23 nucleotides (nt) in length, belong to a set of smal non-coding RNA molecules that were not thought to be functional until the recent decades. miRNAs play important roles in many diseases such as various kinds of cancers and immune disorders. Many studies have focused on the relationship between miRNAs and diseases. miRNAs are significant mediators in human growth and development and in the genesis and development of diseases. Almost 30% of the activity of protein-coding genes is forecasted to be regulated by miRNAs in mammals, and some miRNAs are regarded as potential therapeutic targets for various diseases. In this review, we outline some functions of miRNAs, especialy those related to diseases.
Huandi ZhouXiaohui GeXiaoying Xue
关键词:CANCERDISEASEDRUG
经典Wnt通路与肿瘤放射抗性关系的研究进展被引量:4
2017年
放疗是治疗恶性肿瘤的重要手段,放射抗性是影响放疗疗效的重要因素,也是放疗失败的主要原因之一.近年来经典Wnt信号通路与肿瘤放射抗性的关系,逐渐受到学者的关注.本文检索了近10年来有关经典Wnt信号通路与肿瘤放射抗性的相关研究,并进行综述,对经典Wnt信号通路参与放射抗性形成的分子机制及功能进行系统分析和梳理,力求寻找某些规律和不同作用途径的内在联系,为进一步深入研究寻找有价值的线索.
杨玉周欢娣薛晓英张歌韩雪涛
关键词:肿瘤
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