OBJECTIVE Isofuranodiene(ISO)is a natural product isolated in Chinese herb.Our recent results showed anticancer effect in vitro.In this study,we investigated its live protective effect with a Dgalactosamine/lipopolysacchride(GalN/LPS)induced rat model.METHODS SD rats were treated orally with or without ISO(20 and 50mg·kg-1,ig)for 3d and then treated with GalN/LPS for 8h.The serum were collected and the concentration of aspartate aminotransferase(AST),alanine aminotransferase(ALT),and malondialdehyde(MDA)were determined.The liver injury was examined by H&E staining.The mRNA expression of IL-1β,IL-6 and inducible nitric oxide synthase(iNOS)in liver tissues were determined by realtime PCR.RESULTS Oral administration of ISO(20 and 50mg·kg-1)dramatically inhibited GalN/LPS-induced serum elevation of AST,ALT,and MDA levels.The liver injury was also significantly ameliorated as evidenced by the histological improvement in H&E staining.Furthermore,ISO treatment significantly inhibited GalN/LPS-induced mRNA expression of IL-1β,IL-6,and inducible nitric oxide synthase(iNOS)in liver tissues.CONCLUSION This data showed that ISO has hepatoprotective effect in rats.
