RPMI-1640 was used as the basic culture medium and 30% calf serum was added. Using Berenil, tetracycline,dipterex,tiamulin,imidocarb,florfenicol,ethacri-dine,primaquine phosphate and other drug powder,the drug screening experiment in vitro of Mycoplasma wenyoni was made under the conditions of 37 ℃, 5% CO2. The results showed that the effects of ethacridine was the best ,and that of dipterex and primaquine phosphate were next. The toxicity of dipterex was greater. Berenil, imidocarb and florfenicol were efficient.
