Six alkaloids,2-(1H-indol-3-yl)-4H-benzo[d]oxazin-4-one(1),methyl o-(indole-3-carboxamido) benzoate(2),cyclo(Ile-Leu-Val-Va1)(3),cyclo(Phe-Gly)(4),cyclo(Leu-Leu)(5),and cyclo(Ala-Leu)(6),were isolated from the mangrove endophytic fungus ZSU-H26 from the South China Sea.Their structures were elucidated by spectral data.Indole alkaloids 1 and 2 were both isolated from microorganism for the first time.In the preliminary bioassay,1 and 2 show inhibitory activity on the hepG2 cell line with IC50 values of 20μg/mL and 35μg/mL,respectively.
The secondary metabolites of the fungus Cephalosporium sp.(2090#) are first reported which isolated from the mangrove sample from the South China Sea.Seven compounds are isolated from the mycelium of this strain.Their structures are identified by comprehensive spectroscopic methods.
Eight compounds are isolated from the ferment liquid of mangrove fungus(No.zzf33) by silica gel column chromatography.Their structures are determined to be ramlusin(1),monodictyxanthone(2),8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylic acid methyl ester(3),(E)-dec-2-enedioic acid(4),succinic acid(5),urail(6),thymine(7) and nonacosanoic acid(8) on the basis of comprehensive spectroscopic analysis and chemical method.Compounds 1-3 are isolated from Penicillium sp.for the first time.Compound 8 is first found in marine fungus.In the primary bioassay,compound 2 and 3 exhibit high inhibition to hep2 and hepG2 cells lines.
