An insect excitatory toxin gene from Buthus martensii Karsch(BmKIT)was cloned into the expres-sion vector,pET-28a.BmKIT was expressed as inclusion bodies in Escherichia coli BL21(DE3)hostcells.The authenticity of in vitro expressed protein was confirmed by Western blot.The inclusion bodyprotein band in SDS-PAGE was excised and the protein,BmKIT,was extracted.Polyclonal antibodies tothe purified protein were raised in rabbits.The antibody reacted specifically with the expressed BmKITand was used to quantify its presence in transgenic cotton.
