Objective:To investigate the effect of hepatic ischemia-reperfusion(I/R) on bile canalicular F-actin microfilaments in rats. Methods: A rat model of hepatic ischemia-reperfusion was employed and the ischemia time was 35 min. The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase(AST),γ-glutamyl transferase(GGT) and the level of total bilirubin(TBIL) were measured. Changes in the bile canaliculi were observed by transmission electron microscope. The modification of F-actin microfilaments was quantified by using FITC-Phalloidin and analyzed by confocal laser scanning microscopy imaging. Results:Modifications of F-actin staining were consistent with the observations made by transmission electron microscopy. The staining of F-actin was normal in hepatocytes before reperfusion but decreased significantly after reperfusion, and there was a marked loss of canalicular microvilli after reperfusion, which coincided with abnormal serum GGT and TBIL levels. Conclusion:Reperfusion, not short-term ischemia, induced a disruption of F-actin microfilaments and a loss of microvilli. These modifications could lead to the impaired bile secretion by damaging canalicular contraction, and could be the main mechanisms of cholestasis after hepatic ischemia-reperfusion in rats.
Objective:To investigate whether the method of bridgy duct established between the recipient’s spleen artery and the donor’s gastroduodenal artery could inhibit the apoptosis of liver and bile duct cells caused by hepatic artery ischemia.Methods:Twenty-four mongrel dogs from Xi’an area were used to establish simplified models of dog orthotopic liver transplantation and divided into three groups randomly:HAI group(hepatic artery ischemia group),BBB group(bypassing the blood by a bridgy duct)and control group.After cold perfusion,The samples were collected from liver and bile duct in each group at different time and fixed in glutaraldehyde and 4% polyformaldehyde respectively.At last,the apoptosis of liver and bile duct cells were observed and the apoptotic indexes were calculated.Results:Two hours after cold perfusion,apoptotic phenomenon was common in HAI group,rare in BBB group,while no apoptotic phenomenon was observed in control group.TUNEL staining showed that there was no significant difference in apoptotic index among the three groups immediately after cold perfusion.However,with time going,the apoptotic cells were increased in three groups,and the difference in apoptotic index was significant among three groups(P < 0.01).Conclusion:Bridgy duct of hepatic artery can inhibit the apoptosis of liver and bile duct cells caused by HAI significantly.
