背景与目的:肺癌抑癌基因1(tumor suppressor in lung cancer1,TSLC1)是新近发现的一种肿瘤抑制基因,它在包括前列腺癌在内的多种恶性肿瘤中是失活的。本研究通过将TSLC1转染至人前列腺癌T3B细胞来观察T3B细胞增殖的受抑情况。方法:将克隆有TSLC1全长cDNA的真核重组载体pCI-TSLC1稳定转染至前列腺癌T3B细胞中(实验组)。以转染空质粒pCI-neo的T3B细胞为对照组,野生型T3B细胞为空白组。四甲基偶氮唑蓝法检测细胞增殖,FACSort流式细胞仪检测细胞周期,AnnexinV/PI双染法检测细胞凋亡情况。结果:实验建立了高表达TSLC1蛋白的稳定细胞株。与对照组和空白组相比,实验组细胞生长速度减慢,增殖受到明显抑制,G0/G1期细胞为(80±3)%,高于对照组(66±4)%和空白组(64.2±0.9)%;S期细胞为(11.1±1.3)%,低于对照组(24.0±2.4)%和空白组(26.2±0.6)%,组间差异有显著性(P<0.01),实验组细胞周期发生了明显的G0/G1期阻滞。实验组细胞早期凋亡率,晚期凋亡率和总凋亡率分别为(11.9±0.4)%,(10.2±0.4)%和(22.1±0.6)%,与对照组和空白组细胞相比均明显升高(P<0.01)。结论:TSLC1基因明显抑制T3B细胞增殖,并诱导细胞发生凋亡。
In order to investigate the roles of MTA2 in the pathogenesis of ovarian epithelial cancer, the expression of MTA2 in 4 ovarian cell lines were detected by semi-quantitative RT-PCR and Western-blot assays. MTA2 expression in normal, borderline, benign and malignant epithelial ovarian tissues was immunohistochemically examined. The expression of MTA2 mRNA and protein was detected in all of 4 cell lines of ovarian epithelial cancer. The expression of MTA2 mRNA and protein was higher in strong migration cell lines than in weak migration ones. In borderline and malignant ovarian tissues tested, MTA2 staining was dramatically stronger than in normal and benign tissues (P<0.01). The expression levels in malignant ovarian tissues were significantly higher than that in borderline epithelial ovarian tissues (P<0.01). The expression of MTA2 was correlated with clinical stage, histopathological grade and lymph node metastasis. It was concluded that the high expression of MTA2 was associated with more aggressive behaviors of epithelial ovarian cancer. MTA2 provides a novel indicator of ovarian cancer.
