Removing fruit (RF) and retaining fruit (CK) were carried out during different phenological stages of fruit development onone-year-old shoot of Okubo peach trees [Prunus persica (L.) Batsch.] under preventing exportation of the assimilates tothe non experimental parts of the tree by girdling one-year-old shoot and keeping the same leaves between RF and CK.The results showed that fruit removal significantly decreased net photosynthetic rate (Pn), stomatal conductance (Gs)and transpiration rate (E), but significantly increased leaf surface temperature (TLeaf ) at about midday as compared with CK.Internal CO2 concentration, soluble sugar content, reductive sugar content, starch content except that during the finalrapid fruit growth stage, ADP-glucose pyrophosphorylase and amylase activities in source leaves were not significantlyaffected by fruit removal. There was a significantly positive parabolic correlation between Pn and Gs, and a strongpositive linear correlation between Pn and E. Moreover, Pn increased with increased TLeaf if TLeaf was below 38°C, thendecreased sharply when TLeaf exceeded the above critical temperature for both RF and CK. Pn of RF was lower, however,than that of CK in the same TLeaf , especially if TLeaf exceeded 38°C. It is suggested that the decreased stomatal aperture andincreased TLeaf may be the important mechanism in regulating photosynthesis under a decreased strength of sink demandby RF in fruit trees.