The spectral spread of a graph is defined to be the difference between the largest and the least eigenvalue of the adjacency matrix of the graph. A graph G is said to be bicyclic, if G is connected and ｜E（G）｜ = ｜V （G）｜ ＋ 1. Let B（n, g） be the set of bicyclic graphs on n vertices with girth g. In this paper some properties about the least eigenvalues of graphs are given, by which the unique graph with maximal spectral spread in B（n, g） is determined.
