<正> Bacteriophages infected different serotypes of Klebsiella were isolated from sewage.Among them,aheat-stable polysaccharide depolymerase enzyme which could degrade bacterial exopolysaccharide effec-tively was prepared from the phage infecting Klebsiella K13.Treatment at 60℃ for 30 min could inacti-vate most of the K13 phage,with the titration decreasing from 6.4×10~8 PFU/mL to 1.6×10~6 PFU/mL.However,no obvious loss of phage enzyme activity was found after this treatment.The optimum hydrolytictemperature of phage enzyme was 60℃,with an activity 57% higher than that at 30℃.The addition ofphage enzyme could result in a rapid decrease of viscosity of exopolysaccharide(EPS)solution withinminutes,indicating that K13 phage polysaccharide depolymerase acts as a kind of endo-glyeanohydrolase.HPLC and reducing sugar analysis showed that the hydrolysis of EPS approached approximately the maxi-mum at 4h when the final concentration of phage was 6.0×10~8 PFU/mL.The results showed that Kleb-sieUa K13 phage depolymerase enzyme could be used as a good tool for the preparation of EPS oligosac-charide.
