The samples of pueraria lobata ohwi were digested by HNO3 + HClO4.contents of the next trace elements Fe,Zn,Cu,Mn,Ca,Mg,Co,Cr,Ni,Cd,Pb and Al in the bloom,base,stem,and leaf of Impatiens balsamina have been determined by Flame Atomic Absorption method.The result show that the recovery is about 97.5%-103.2%,and the RSD<2.7%,with good accuracy and precision.The order of the content of the trace elements of high to low is as follows:leaf>stem>bloom>base.The importance of essential trace elements Zn,Fe,Mn,Cu for human each has a higher content.In addition,toxic trace elements such as lead,arsenic and cadmium are also found.
To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.
Hesperidin and naringoside in xiangsha yangwei pill has been determined by the HPLC,in which the DiamonsilTMC18(4.6 mm×150 mm,5 μm)was used as the column at UV 283 nm and the CH3CN-H2O solution(22∶78) was made use of mobile phase,in which H3PO4 was used as agent to regulate pH(3.5),the flow rate is 1.2 mL·min-1,there was a fine separated degree between the pending component and other component and the lineal range of Hesperidin and naringoside is 0.25μg^1.49 μg and 0.023μg^0.14 μg respectively.The average recoveries of Hesperidin and Naringoside were 99.3% and 102.6% respectively with RSD being 1.2% and 2.5%.The result can give a simple,effective method for controlling the quality of xiangsha yangwei pill.
