Objective: To evaluate the frequency of MSI in epithelial ovarian tumors and its relationship with clinicopathologic features. Methods: Ninety fresh specimens of epithelial ovarian tumors, including 74 primary and 16 secondary tumors, were collected. Microsatellite analysis was carried out using 5 mono- and dinucleotide markers from the National Cancer Institute Consensus Panel by fluorescence-labeled polymerase chain reaction. Results: Of 90 epithelial ovarian tumors analyzed, 18 demonstrated a high level of microsatellite instability (MSI-H), 30 demonstrated a low level of microsatellite instability (MSI-L), and the remaining 42 exhibited microsatellite stability (MSS). Frequency of microsatellite instability (MSI) at loci BAT-25 was higher than that at any other loci. No correlation was found between MSI level and patient age, tumor type, tumor differentiation (P>0.05). But the microsatellite instability-high phenotype correlates with clinical stage.It tended to occur more frequently in early-stage tumors (P=0.03). Conclusion: The frequent MSI in epithelial ovarian tumors suggests that it is an early event to involve in the development of epithelial ovarian tumors.
This paper investigates the equivalent model for the friction boundary condition between blank and holder during sheet metal forming simulation, and proposes an equivalent algorithm, the thickness threshold value algorithm, for the first time based on thickness weighting. This algorithm assumes that the holder clearance is uniform during sheet metal forming; and the main reason for the unevenness of friction force distribution under the holder is that the uneven deformation of the blank leads to its uneven thickness distribution, which makes the local pressure on the blank distributed unevenly. The algorithm proposed in this paper can effectively simulate the influence of the unevenness on the forming process. Validity of this algorithm is verified by a comparison between the simulation results and the experimental ones for the drawing process of a car spring base.
