目的研究负载不同转移潜能人肝癌树突状细胞中β-中心体肌动蛋白(β-centractin)表达的差异及其与树突状细胞(DC)表型、功能变化的关系。方法不同转移潜能肝癌分别负载 DC,无负载 DC 为对照。流式细胞仪检测表型,混合淋巴细胞反应检测 DC 的功能;用 Western 印迹和细胞免疫化学方法对β-centractin 进行验证和定位。结果负载组 DC 其 HLA-DR、CDS0、CD83和 CD86均明显高于无负载组 DC(P<0.05),负载组中 DC 的 HLA-DR、CD1a 差异无统计学意义,但负载高转移肝癌 DC 的 CD86表达和混合淋巴细胞反应(MLR)能力明显低于其他负载组 DC (均 P<0.05)。Western 印迹和细胞免疫化学方法发现β-centractin 在无负载 DC 中不表达,在负载高转移肝癌 DC 中表达低于其他负载组 DC,与其在双向电泳图谱中表现一致。结论β-centractin 在负载高转移肝癌DC 中表达的下调与 DC 表型和功能缺陷相关,可能是造成肝癌免疫逃逸的机制之一。
Objective To investigate the relationship between the distribution and quantity of dendritic cells (DCs) and memory T lymphocytes in the tumor site and the prognosis of hepatocellular carcinoma (HCC).Methods Hematoxilin and eosin staining was done on 123 specimens of pathologically proven HCC to observe the infiltration of lymphocytes. Immunohistochemistry was used to count the S-100 protein positive DCs and CD45RO antigen positive memory T cells in ten high power fields within a specimen. Double immunohistochemical staining with double enzyme renovated by microwave for S-100 protein and CD45RO antigens was used to observe relationship between the positivity of S-100 protein and positivity of CD45RO antigen. The specimens were divided into 4 groups according to the averaged numbers of DCs and memory T cells: group I with the DCs and T cells both ≥average numbers, group II with the DCs ≥T cells and T cells 0.05),the number of DCs in the cancerous node (DC≥25/10high power field) was positively correlated with the tumor-free survival rate (P=0.005) and the number of T cells in the cancerous node was positively correlated with the tumor-free survival rate (P=0.003). Double staining method of immunohistochemistry showed that in the cancerous nodes the number of CD-100 positive DCs and the number of CD45RO positive memory T cells was significantly correlated (r=0.531, P=0.000). A greater number of DCs in the cancerous nodes showed a longer tumor-free survival rate of the patients (P=0.000 between group Ⅰ and group Ⅳ, P=0.009 between group Ⅱ and group Ⅳ, and P=0.545 between group Ⅰ and group Ⅱ).Conclusion The tumor-free survival rate of the patients who had more intratumoral DCs is higher,which indicates
