The micron-sized microspheres with superparamagnetic property were synthesized with vinyl acetate and divinylbenzene by microsuspension polymerization.After the complete alcoholysis,these hydroxyl-functionalized microspheres were activated by divinylfone and modified with mercaptoethanol to prepare the thiophilic magnetic adsorbent,which was used to specifically isolate immunoglobulin G (IgG) from human serum.This thiophilic magnetic adsorbent performed an evident salt-dependent adsorption behavior for IgG.Due to their salt-promoted adsorption towards IgG under high salt concentration,the absorbed antibodies could be extracted in low salt concentration with high purity.
