Drought stress is one of the major factors affecting in wheat yield and grain quality. In order to investigate how drought stress might infl uence wheat quality during grain fi lling, three wheat cultivars Gaocheng 8901, Jagger and Nongda 3406 were subjected to drought stress during the grain fi lling stage. Neither globulin and glutenin, nor the relative percentage of amylose signifi cantly changed following drought treatments, whereas albumin and gliadin concentrations did. The SDS-sedimentation, which has a strong linear correlation with wheat baking quality was markedly decreased following drought stress. These results indicated that drought had an adverse effect on wheat quality. In order to investigate the protein complexes in the wheat fl our, the data from native PAGE and SDS-PAGE were combined and a total of 14 spots were successfully identifi ed, and of these eight protein types were determined to be potential complex forming proteins.
With the increase in consumer demand,wheat grain quality improvement has become a focus in China and worldwide.Transcriptome analysis is a powerful approach to research grain traits and elucidate their genetic regulation.In this study,two cDNA libraries from the developing grain and leaf-stem components of bread wheat cultivar,Nongda211,were sequenced using Roche/454 technology.There were 1061274 and 1516564 clean reads generated from grain and leaf-stem,respectively.A total of 61393 high-quality unigenes were obtained with an average length of 1456 bp after de novo assembly.The analysis of the 61393 unigenes involved in the biological processes of the grain showed that there were 7355 differentially expressed genes upregulated in the grain library.Gene ontology enrichment and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that many transcription products and transcription factors associated with carbohydrate and protein metabolism were abundantly expressed in the grain.These results contribute to excavate genes associated with wheat quality and further study how they interact.
Liu WEIZhihui WUYufeng ZHANGDandan GUOYuzhou XUWeixia CHENHaiying ZHOUMingshan YOUBaoyun LI
