Antioxidant enzyme activity,photosynthetic pigment content,and free malondialdehyde(MDA),as well as flavonoid content and the key enzyme activity in the flavonoid pathway were determined in two desert shrubs,Caryopteris mongolica Bunge and Reaumuria soongorica(Pall.)Maxim.under drought stress.The free MDA content was enhanced during the experimental period,which may be an indicator of oxidative stress.Superoxide dismutase(SOD),peroxidase(POD),and ascorbate peroxidase(APX)activities in C.mongholica showed a significant increase during the experiment,but catalase(CAT)activity was slightly decreased.On the other hand,POD and APX activities showed a significant increase and SOD and CAT activity data had no significant changes in R.soongorica.APX,SOD,and CAT activities were higher in R.soongorica than in C.mongholica,but MDA content was lower,indicating that the lower values of MDA were attributed to higher activities of antioxidant enzyme in R.soongorica.Chlorophyll content decreased significantly in the two shrubs during the experiment,which indicated that there was a photopro-tection mechanism through reducing light absorbance by decreasing pigments content.Caretonoids content increased in C.mongholica and decreased in R.soongorica.The ratio of Chla/Chlb decreased significantly but caretonoids/Chl revealed a sig-nificant increase in the two shrubs,which could be explained as no decrease of peripheral light-harvesting complexes and a higher tolerance to drought.Total flavonoid content and the activities of phenylalanine ammonialyase(PAL)and chalcone isomerase(CHI)showed different changes between C.mongholica and R.soongorica after treatment.These values decreased in R.soongorica and increased in C.mongholica except for PAL activity.However,anthocyanin content increased in the two shrubs,indicating that there was a different regulation response in the flavonoid pathway in the two shrubs under drought stress,and an-thocyanin should be an important antioxidant both in the shrubs.Our results demonstrated the different r
The seedlings of Halocnermum strobilaceum were cultivated in 0.5%hoagland nutrient solution containing 0.0%,0.9%,2.7%and 5.4%of NaCl as well as composite salt(Na^+,Ca^(2+),K^+,Si^(4+)) for 20 days;all the contents are in weight ratio.Succulent level,inorganic ions(Na^+,K^+),organics such as betaine,proline,malondialdehyde,and antioxidant enzyme activities including superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),betaine aldehyde dehydrogenase(BADH) were measured to reveal its salt tolerance mechanism.When the composite salt concentration reaches 5.4%,SOD activity level,and MDA content is five times the control group;when it reaches 2.7%,the succulent level of seedlings,and the content of K^+ in roots is nearly two times the NaCl treatment;the dry weight is more than three times the control group;with the NaCl treatment,MDA is three times the contrast;when the salt concentration is 2.7%,POD reaches the maximum.Results indicate that Si^(4+),K^+,and Ca^(2+) from composite salt in the roots of H.strobilaceum improved the water-holding capacity.The activities of antioxidant enzyme were raised by the accumulation of proline and betaine,which increased the salt tolerance.The absorption of K^+ promoted the high ratio of K^+/Na^+ and alleviated the damage of cell membranes of H.strobilaceum,which is associated with osmotic contents such as betaine and proline.
