Using the optical coherence tomography(OCT) images,the optical attenuation coefficients(μ_t) of human Laogong acupoint and non-acupoint tissues are measured after empty irradiation and 808-nm 100-mW irradiation for 10 min in vivo non-invasively.The results show that there is no significant difference ofμ_t of Laogong acupoint and non-acupoint tissues after empty irradiation.However,there are significant differences ofμ_t between Laogong acupoint and non-acupoint tissues after laser irradiation at the power of 100 mW(statistical definition of probability p<0.01).The results of the pilot study indicate that the OCT could distinguish the acupoint from the surrounding tissues after irradiation with laser in vivo non-invasively.