A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercritical carbon dioxide,and HPLC was used to analyze the transferred sample.The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum.The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined,which accorded with the result obtained with off-line extraction.Using this system,the whole process can be monitored and the optimum extraction time can be determined with single run.In addition,it has many other advantages,such as low sample loading,high sensitivity and accuracy,short analysis time,avoiding sample pollution and easily operation etc.The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.
目的 建立反相高效液相色谱法对五味子中的保留木脂素成分同时进行含量测定。方法 反相高效液相色谱法,色谱柱为Inertsil ODS-3 column(250 mm×4.6 mm i.d.,5μm),采用梯度洗脱的方式,流动相:A 甲醇,B 水。梯度洗脱:0-16 min VA : VB=70:30,16-40 min VA:VB=100:0。结果 五味子中四种木脂素与周围未知成分达到基线分离,四种木脂素线形关系良好,相关系数r>0.999。结论 此法简单、灵敏、快速,为分析五味子及其相关制剂中主要单体木脂素提供了一种可行质量控制方法。
