Objective The adverse effects of 5-fluorouracil(5-FU) are well recognized,especially hepatotoxicity and nephro...
Zhiyun Cao~1,Lianming Liao~1,Xuzheng Chen~1,Lan Lan~1,Haixia Hu~1,Zhizhen Liu~1,Liwu Chen~1, Suping Huang~1,and Jian Du~1 (Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine, Fuzhou 350122)
Objective:To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine(CM)of Fuzheng Guben(扶正固本,supporting the healthy energy and strengthening the body's resistance to pathogens),the effects of Fuzheng Yiliu Decoction(扶正抑瘤方,FYD),a typical prescription of Fuzheng Guben,on proliferation and apoptosis of hepatoma cells in vitro were observed by two methods with serum pharmacology and traditional pharmacology,respectively.Methods:HepG2 cells were treated with FYDcontaining serum or crude FYD extract in vitro.The proliferation rate was determined by methyl thiazolyl tetrazolium(MTT)assay.Cell cycle and apoptosis rate was performed by flow cytometry.And the levels of interleukin-2(IL-2)and tumor necrosis factor α(TNF-α)in FYD-containing serum were detected by radioimmunoassay.Results:FYD-containing serum remarkably inhibited proliferation and induced apoptosis of hepatoma cells at least by promoting the production of IL-2 and TNF-α in vivo.On the contrary,crude FYD extract promoted the proliferation and did not induce cell apoptosis.Conclusion:The results by serum pharmacology were accordant with those of our previous animal and clinical trials which indicates that serum pharmacology is a reasonable and feasible method for the evaluation of the antitumor effect of herbs of Fuzheng Guben.
