In this paper, the effects of methmidophos and phoxim with different concentrations and schedule on soluble protein content and chlorophyl a (Chla) content in Porphyra haintanensis were studied under experimental and ecological conditions. The results showed as follows: (1) there were some time-effect and dose-effect relationships between the pollutants and Chla contents. The Chla content of most of groups decreased with the increasing of dose and prolonging of the exposure time. In the same concentration, the toxicities of phoxim to Chla content was stronger than that of methmidophos. (2) All concentration groups in this study didn’t show the significant time-effect and dose-effect relationships between pollutants and the soluble protein. And there was no significant difference of the soluble protein content among various groups.