Objective:To observe the effect of electroacupuncture at"Zusanli(ST36)"on 5-fluorouracil(5-FU)-induced renal injury in colorectal cancer-bearing mice,and to further investigate its effects on oxidative stress,inflammatory responses,and cell apoptosis in mouse renal tissue.Methods:Thirty-five male BALB/c mice were randomly divided into five groups of seven mice each,namely the control,CT26,5-FU,sham point(SP),and ST36(which received EA at the"ST36")groups.With the exception of the control group,each group was subjected to establishment of a subcutaneous implantation tumor model using the murine CT26 colorectal cancer cell line.Once the models were successfully established,the 5-FU,SP,and ST36 groups received 5-FU injection solution intraperitoneally at a dose of 5 mg/mL once every three days over a 21-day period.Mice in the SP and ST36 groups additionally received an EA intervention after each intraperitoneal 5-FU injection.EA were performed on mice of the SP group at bilateral sham acupoints and on mice of the ST36 group at the bilateral"ST36"using the continuous wave mode at a frequency of 2 Hz for a duration of 5 min,intervention was administered once every two days for a duration of 21 days.Samples were collected from the mice at the end of the experiment.The pathological morphology of the renal tissue was observed using hematoxylin and eosin(HE)staining;the contents of creatine(Cre),blood urea nitrogen(BUN)and malondialdehyde(MDA),and superoxide dismutase(SOD)activity were measured using biochemical assays;the expression and nuclear translocation of nuclear factor kappa B p65 subunit(NF-kB p65)were measured by immunofluorescence;the expression levels of tumor necrosis factor alpha(TNF-α),interleukin-6(IL-6),and interleukin-1β(IL-1β)in serum were measured by ELISA;cell apoptosis in renal tissue was detected using the TUNEL assay;and the expression levels of the anti-B-cell lymphoma/leukemia 2(Bcl-2),Bcl-2-associated X protein(Bax),cleaved caspase-3,cleaved caspase-9,and cytochrome C(cyt c)in renal tissue we
