In this paper,we studied the effects of the adding time of alkane on the expression of P450 and production of dicarboxylic acid.A novel fermentation process,in which no or a little alkane was added to make the cells growing more quickly during the growth stage,followed by the addition of alkane to induce cytochromes P450 for 6～8 hours,was established.The results showed that the new process was much better than the old process on inductivities of cytochromes P450 and production of dicarboxylic acid.The new process improved nearly 14.56% expression of P450 and 14.15% production of dicarboxylic acid.
