[Objective] The aim was to explore the distributions of main microorganisms in constructed wetland of Iris pseudacorus L.and their function and position in wastewater treatment.[Method]Distributions of main microorganisms in the upstream vertical-flow constructed wetland of Iris pseudacorus was studied by training and observing 4 kinds of microorganisms.[Result]The total number of microorganisms on root surface in unit area was 15.32 times as many as that on filler surfacein,among them bacteria and actinomycete were 17.14 times and 19.84 times as many as those on filler surfacein,respectively.[Conclusion]The distributions of microorganisms are significantly different in different parts of wetland system,obviously on root surface.Bacteria are the main populations of biological wastewater treatment.