应用高效液相色谱法／二极管阵列检测器同时测定了飞机草中木犀草素、槲皮素和山柰酚的含量。色谱柱HiQ sil C18W柱（4．6mm×25cm，5μm），流动相V（甲醇）：V（水）：V（磷酸）≈50：49．8：0．2，检测波长槲皮素254nm，木犀草素和山柰酚360nm，温度30℃，流速1mL／min，进样量10μL。确定了以超声波提取法制备飞机草分析样品的方法：溶剂为体积分数85％的乙醇，液固比为10：1（mL／g），提取时间为1h。结果表明，3种黄酮在0．01×10^-3－0．10×10^-3g／mL范同内呈现良好线性关系（R^2〉0．9990），平均加样回收率分别为99．6017％、99．0326％和99．4508％，RSD〈2％。该方法操作简便、准确度高，可快速测定飞机草中木犀草素、槲皮素和山柰酚3种物质的含量。
为了建立一种高效提取沙棘果中总黄酮的方法,对匀浆法提取沙棘果中总黄酮的工艺进行了研究,确定了最佳的工艺参数:提取原料为含水率85%的沙棘果,溶剂为85%的乙醇,匀浆时间10 m in,液固比为5∶1(mL∶g)。将该法与常规的回流提取法进行了比较。结果表明,在优化的条件下,匀浆法对沙棘果总黄酮的提取率为0.76%,与回流提取相当。匀浆提取法所用提取时间短,提取溶剂用量少,该法是一种高效、快速提取沙棘果总黄酮的方法。
This paper presents a vacuum-cavitated extraction method for glycyrrhizic acid(GA) extraction from supercritical extraction residue.The optimum extraction conditions are as follows:0.3% ammonia solution as the solvent,the ratio of solvent to solid 10∶[KG-*3/5]1(mL/g),vacuum-cavitated time 15 min,and the crude (extract) yeild is 30.3%,in which the content of GA is 6.6%,so the GA extraction yeild is 2.0%.There are three extraction methods:soxhlet,ultrasonic wave,and vacuum-cavitated extraction methods.The results show that the extraction yield of GA by vacuum-cavitated extraction method is 1.6 times that by soxhlet extraction method and 1.2 times that by ultrasonic.At the same time,the vacuum-cavitated extraction method shows many advantages,including lower temperature,less solvent,shorter extraction time,higher extraction yield,and higher GA content in the crude extract.The vacuum-cavitated extraction method is new and highly(efficient),which can be utilized in the pharmaceutical industry.
