In this article, the preparation of core-shell fluorescent dye nanoparticles doped with rhodamine B isothiocyanate by using novel method is reported, which involves synchronous hydrolysis of tetraethoxysilane(TEOS) in water in oil microemulsion with the combination of N-(β-amimoethyl)-γ-amino propyltriethoxy-silane(AEAPS) and rhodamine B isothiocyanate(TRITC). This method conquers the disadvantages of traditional preparation method about organic dye doped core-shell nanoparticles such as the leakage of fluorescent dyes and the low encapsulation efficiency of fluorescent dyes. The fluorescent nanoparticle prepared by using the novel method have the advantages such as high fluorescence and good fluorescent stability, which are hopeful to be applied in the field of biological labelling.
