Twelve known compounds, colchicine, 2-demethylcolchicine, 2-demethyldemecolcine, 2-demethylcolchifoline, 2-demethyl-lumicolchicine, lumicolchicine, demecolcine, luteolin, apigenin, n-hentriacontane, n-triacontanol and sitosterol were isolated from cormsand flowers of colchicum autumnale Liliaceae which was introduced from Europe and cultivated in Zhaotong Yunnan, China The contents ofcolchicine in corms and flowers of colchicum autumnale were 0.015% and 0.04% respectively, and the content of demecolcine in corms was0.043%.
Chlorothalonil 1 was treated with anhydrous KF in DMF at 110 ℃ to give the corresponding crystalline fluorine-containing 1,3-benzenedicarbonitrile 2. KF was used in an equivalent to the number of chlorine atoms to be substituted. Compound 2 was subsequently reacted with ammonia to yield compound 3. In addition, halobenzamide 4 were refluxed with oxalyl chloride in anhydrous 1,2-dichloroethane(DCE) to yield benzoylisocyanates 5. Finally, seven novel BPUs chitin inhibitors 6a—6g were synthesized via the selective reaction of compound 3 with benzoylisocyanate derivatives 5, the total yield is over 30%—50%.
