The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis larvae was tested. The result showed that the optimum culture medium was PPDA,the optimum temperature for the mycelium growth and spore germination was 26 ℃,and A. glabripennis larvae had the highest mortality from the fungus at this temperature. The optimum RH for the mycelium growth ranged from 95%to 100%. The speed and rate of spore germination increased with increasing of RH. The larvae died from the fugus most quickly and the mortality reached the highest under 100% RH.
