[Objective] The aim was to build spectral diagnosis model for nitrogen nutrition in rice leaf.[Method] Through determination of transmission,absorption spectra and nitrogen content on rice leaves of different rice varieties(Xiangyou 109 and Shanyou 98),different growth periods and different nitrogen levels,we analyzed the correlation between leaf spectra and their nitrogen contents,and built prediction model of spectral index for nitrogen content in rice leaf.[Result] The detection precision of this model was above 80% that can be used on diagnosing of rice nitrogen nutrition.[Conclusion] The study provided evidence for monitoring nitrogen nutrition status of rice.