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张晓丹

作品数:7 被引量:0H指数:0
供职机构:北京大学更多>>
发文基金:国家教育部博士点基金国家自然科学基金更多>>
相关领域:医药卫生文化科学更多>>

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生物材料作为相互作用相的毛细管及其制备和应用
本发明提供了一种带有柱筛并以生物材料作为相互作用相的毛细管、其制备方法以及通过毛细管电泳方法在研究生物大分子与活性配体或化合物相互作用以及药物筛选中的用途。针对某些生物活性大分子从细胞或离体生物组织中分离纯化难度大且会引...
凌笑梅武瑞君李晨朱凯张晓丹张素芳任进宇刘燕萌杨东辉叶敏
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审美精神的上行性
张晓丹
关键词:审美活动
生物材料作为相互作用相的毛细管及其制备和应用
本发明提供了一种带有柱筛并以生物材料作为相互作用相的毛细管、其制备方法以及通过毛细管电泳方法在研究生物大分子与活性配体或化合物相互作用以及药物筛选中的用途。针对某些生物活性大分子从细胞或离体生物组织中分离纯化难度大且会引...
凌笑梅武瑞君李晨朱凯张晓丹张素芳任进宇刘燕萌杨东辉叶敏
Rapid screening of anti--HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV--1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresis
2015年
HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the entry process of HIV-1 into cells. The development of new inhibitors is essential since the viral enzyme reverse transcriptase (RT) is one of the first targets of antiretroviral therapy. It has been reported that a variety of natural plants, such as Artemisia rupestris L., have anti-viral pharmacological activity, and they might be the potential inhibitors of RT or V3 loop of gpl20 against HIV-1. RIQRGPGRAFVT1GK (R15K), the relatively conserved region of V3 loop, can be used for binding research. In this work, we analyzed the interactions between different extracts from Artemisia rupestris L. and R15K by affinity capillary electrophoresis (ACE). Moreover, we analyzed the interactions between different extracts from Artemisia rupestris L. and RT by capillary zone electrophoresis (CZE). Our data showed that the chloroform extract ofArtemisia rupestris L. was active among the different plant extracts, which was consistent with previous studies. Taken together, our study provided a rapid screening method to seek anti-HIV ingredients in natural plants' extracts.
赵怡然李中杰姜勇张晓丹凌笑梅
关键词:HIV
A new method of immobilizing HEK293 cells on the inner wall of a capillary column as stationary phase for drug screening
2016年
It is a new strategy to immobilize cells on the inner wall of a capillary column and use affinity capillary electrophoresis(ACE) to study receptor-ligand interactions or to screen natural products and compounds synthesized by combinatorial chemistry. In this paper, we developed a new method of immobilizing HEK293 cells on the inner wall of a capillary column. Four important experimental conditions were optimized, including cell injection density, PLL concentration, cell culturing time and sterile processing method. Immobilized cell-coated capillary columns prepared under the optimized experimental conditions exhibited good uniformity, stability and durability, which were suitable for capillary electrophoresis. The method could also be used to immobilize HEK293 cells over-expressing certain membrane receptors on the inner wall of a capillary. In this way, cell-coated capillary columns could be applied to ACE drug screening targeting certain membrane proteins.
张晓丹李晨朱凯刘燕萌武瑞君任进宇凌笑梅
仿生拮抗血管内皮细胞与白细胞粘附毛细管及其电泳方法及用途
本发明提供了一种仿生拮抗血管内皮细胞与白细胞粘附毛细管及其电泳方法及用途,包括将细胞贴附培养于毛细管内壁作为固定相,在流动相中加入拮抗受体‑配体相互作用的化合物,并利用该毛细管进样分析细胞的方法,利用此法可以筛选得到具有...
凌笑梅赵怡然贾凌晗李美娜张晓丹李庆李中军
仿生拮抗血管内皮细胞与白细胞粘附毛细管及其电泳方法及用途
本发明提供了一种仿生拮抗血管内皮细胞与白细胞粘附毛细管及其电泳方法及用途,包括将细胞贴附培养于毛细管内壁作为固定相,在流动相中加入拮抗受体‑配体相互作用的化合物,并利用该毛细管进样分析细胞的方法,利用此法可以筛选得到具有...
凌笑梅赵怡然贾凌晗李美娜张晓丹李庆李中军
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