FM4-64,a kind of membrane-selective fluorescent dye,was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici.The effect of FM4-64 on hypha was observed under confocal microscope.The results indicated that the plasma membrane,speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 μmol/L.The optimal dye concentration was 6 μmol/L for the both pathogens.At the same concentration of FM4-64,the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times.The staining of plasma membrane and vesicles should be completed in 5 minutes,as other organelles can be stained after 10 minutes.
