Detection of deoxyribozyme （DNAzyme） cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8- 17deoxyribozyme （8-17DNAzyme） against hepatitis C virus RNA （HCV-RNA） was designed and the cleavage process was studied in real time. ？2009 Ke Min Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.
Xiang Xian Meng Xiao Hai Yang Ke Min Wang Wei Hong Tan Qiu Ping Guo
