A simple and accurate high-performance liquid chromatography（HPLC）method to generate the fingerprints of crude Pu-erh tea（CPT）and ripened Pu-erh tea（RPT）is described.A suitable chromatographic system was established using a linear gradient elution with acetonitrile and water containing 0.6% formic acid as the mobile phase and a detection wavelength of 300 nm.HPLC analysis identified 24 common peaks among RPT and 21 common peaks among CPT.This study revealed that crude Pu-erh tea and ripened Pu-erh tea contained some similar major constituents,but with distinctive peaks,which may be caused by the difference in the fermentation process.This HPLC fingerprint method could be used to evaluate and authenticate crude Pu-erh tea and ripened Pu-erh tea.
Fifteen compounds were isolated from the processed seeds of Strychnos nux-vomica and were identified as follows：strychnine（1）,brucine（2）,pseudostrychnine（3）,pseudobrucine（4）,secoxyloganin（5）,caffeic acid（6）,p-hydroxybenzoic acid（7）,p-hydroxyphenylacetic acid（8）,uvaol（9）,stigmasta-7,22,25-triene-3-ol（10）,lupeol（11）,11-oxo-α-amyrin palmitate（12）,catechol（13）,maltol（14）,adenosine（15）.Compounds 5-15 were isolated from genus Strychnos for the first time.
